Perfusion needle, tubing and T-connector assembly

Quality standard

Assembly comprising a perfusion needle, connecting tubing and a T-connector; the source does not report needle gauge, tubing diameter or materials.

Use information

Used for left ventricular cannulation, connecting prewash and fixative solutions to the perfusion tubing through a T-connector.

References

1. protocols.io v1; step protocolsio-8435167; verbatim source: a needle connected to tubing (T-connector to prewash and perfusion solutions)

Used in these protocol BOMs

• Intracardiac perfusion fixation of experimental mice: preparation of tissue specimens for anatomical studiesprotocol #183 · revision 5

  Source version：protocols.io · v1 · Janet R Keast; Luke Bowden; Peregrine B Osborne · CC BY

  Cell and Tissue Fixation

  This reagent's uses & amounts

  • Thoracotomy and left ventricular cannulation · Used for left ventricular cannulation, connecting prewash and fixative solutions to the perfusion tubing through a T-connector. · Not reported in the source · Assembly comprising a perfusion needle, connecting tubing and a T-connector; the source does not report needle gauge, tubing diameter or materials.

  Associated models: 33 protocol→model record(s)3 verified · 0 candidate

  Model → research topic：3 verified · 0 candidate。These statuses apply to individual relationships, not to this reagent.

  Recorded use and applicability limits

  • Protocol → model：C57 mouse MCAO suture model；Protocol → model：5xFAD transgenic model；Protocol → model：3xTg-AD transgenic model：

    Adult mouse brain tissue was fixed by cardiac perfusion and preserved, providing samples with well-preserved morphology for anatomical observation and subsequent microscopic examination.

  • Protocol → model：C57 mouse MCAO suture model；Protocol → model：5xFAD transgenic model；Protocol → model：3xTg-AD transgenic model：

    Age：adult

  • Protocol → model：C57 mouse MCAO suture model；Protocol → model：5xFAD transgenic model；Protocol → model：3xTg-AD transgenic model：

    Tissue：brain

  • Model → research topic：C57 mouse MCAO suture model → ischemic stroke：

    Transient MCAO reperfusion model in C57 mice for the study of ischemic brain injury.

  • Model → research topic：5xFAD transgenic model → Alzheimer disease：

    The 5xFAD transgenic model is used for research on Alzheimer's disease-related amyloid pathology and neurodegenerative changes.

  • Model → research topic：3xTg-AD transgenic model → Alzheimer disease：

    The 3xTg-AD transgenic model is used for research on Alzheimer's disease-related amyloid and tau pathology.

  Show 3 model association(s)

  C57 mouse MCAO suture model · Verified applicability

  This protocol's applicability to this model

  Status：Verified applicability· Evidence type：Literature evidence· Source：Keast JR, Bowden L, Osborne PB. Intracardiac perfusion of experimental mice with fixative for anatomical studies. protocols.io 227096 v1 (2026-09-10), DOI:10.17504/protocols.io.bp2l6z7xdgqe/v1; the source protocol is explicitly suitable for anatomical study preservation of organs, ganglia, spinal cord, or brain tissue in adult mice

  Age：adultTissue：brain

  Adult mouse brain tissue was fixed by cardiac perfusion and preserved, providing samples with well-preserved morphology for anatomical observation and subsequent microscopic examination.

  Related research topics: 1 (1 verified · 0 candidate)

  • · ischemic stroke · Verified applicability

    This model's applicability to this disease

    Status：Verified applicability· Evidence type：Literature evidence· Source：PMID:37786756; https://pubmed.ncbi.nlm.nih.gov/37786756/; DOI:10.1002/ibra.12128

    Transient MCAO reperfusion model in C57 mice for the study of ischemic brain injury.

  5xFAD transgenic model · Verified applicability

  This protocol's applicability to this model

  Status：Verified applicability· Evidence type：Literature evidence· Source：Keast JR, Bowden L, Osborne PB. Intracardiac perfusion of experimental mice with fixative for anatomical studies. protocols.io 227096 v1 (2026-09-10), DOI:10.17504/protocols.io.bp2l6z7xdgqe/v1; the source protocol is explicitly suitable for anatomical studies and preservation of organs, ganglia, spinal cord, or brain tissue in adult mice

  Age：adultTissue：brain

  Adult mouse brain tissue was fixed by cardiac perfusion and preserved, providing samples with well-preserved morphology for anatomical observation and subsequent microscopic examination.

  Related research topics: 1 (1 verified · 0 candidate)

  • · Alzheimer disease · Verified applicability

    This model's applicability to this disease

    Status：Verified applicability· Evidence type：Literature evidence· Source：PMID:17021169; https://pubmed.ncbi.nlm.nih.gov/17021169/; DOI:10.1523/jneurosci.1202-06.2006

    The 5xFAD transgenic model is used for research on Alzheimer's disease-related amyloid pathology and neurodegenerative changes.

  3xTg-AD transgenic model · Verified applicability

  This protocol's applicability to this model

  Status：Verified applicability· Evidence type：Literature evidence· Source：Keast JR, Bowden L, Osborne PB. Intracardiac perfusion of experimental mice with fixative for anatomical studies. protocols.io 227096 v1 (2026-09-10), DOI:10.17504/protocols.io.bp2l6z7xdgqe/v1; the source protocol is explicitly suitable for anatomical studies and preservation of organs, ganglia, spinal cord, or brain tissue in adult mice

  Age：adultTissue：brain

  Adult mouse brain tissue was fixed by cardiac perfusion and preserved, providing samples with well-preserved morphology for anatomical observation and subsequent microscopic examination.

  Related research topics: 1 (1 verified · 0 candidate)

  • · Alzheimer disease · Verified applicability

    This model's applicability to this disease

    Status：Verified applicability· Evidence type：Literature evidence· Source：PMID:12895417; https://pubmed.ncbi.nlm.nih.gov/12895417/; DOI:10.1016/s0896-6273(03)00434-3

    The 3xTg-AD transgenic model is used for research on Alzheimer's disease-related amyloid and tau pathology.

Reference, supply and purchase path

No verified catalog mapping exists for this reagent; reference status: unknown.

No listed sale configuration is published here. This states neither discontinuation nor current stock.