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Technical services

Histopathology

Examine tissue pathology with section staining and immunohistochemistry.

Histopathological section staining

Observe tissue morphology and lesions, including plaque section staining in atherosclerosis research.

IHC

Immunohistochemistry to examine the distribution of target markers in tissue.

Molecular assays

Explore gene expression and protein measures with qPCR, ELISA and WB.

qPCR

Quantitative PCR analysis to measure target gene expression.

ELISA

Enzyme-linked immunosorbent analysis to measure target proteins in samples.

Western blot (WB)

Western blot analysis to examine target protein expression.

Multiomics

Explore molecular changes in tissues and cells through transcriptomic data in model research.

Single-cell transcriptomics

Analyze gene expression at single-cell resolution, including hippocampal tissue in Alzheimer’s disease model research.

Sample requirements, assay scope, deliverables and current availability are confirmed for each research project.

Experimental methods

Experimental protocols

Protocols apply a method to a defined measurement, with samples, procedures and quality controls. Available services are linked separately.

  • 天狼星红（Picrosirius red）胶原染色

    Version 3

    This protocol adopts the protocols.io Picrosirius red staining for histology v1. Formalin-fixed paraffin-embedded sections are dewaxed and rehydrated, optionally pre-treated with phosphotungstic acid and counterstained with Weigert haematoxylin, then stained with 0.1% Sirius Red F3BA in saturated picric acid, differentiated with acetic acid, dehydrated, cleared and mounted. Collagen appears red in bright field and birefringent under polarised light. The record describes staining and microscopic observation, not a complete fibrosis area or stage quantification.

  • cDNA 模板的实时定量 PCR（qPCR，SYBR Green 分支）

    Version 3

    This protocol adopts the SYBR Green branch of the protocols.io qPCR v1 record. The input is pre-generated cDNA (10 ng per well); RNA extraction and reverse transcription are not included. PowerUp SYBR Green Master Mix and forward/reverse primers are used on a LightCycler 480 with 50˚C for 2 min, 95˚C for 2 min, and 40 cycles of 95˚C for 15 s plus 60˚C for 1 min. CT values are read and analysed by the ΔΔCT method. No specific genes, primer sequences or disease-model readouts are asserted.

  • 小鼠胰岛素耐量试验（ITT）

    Version 2

    This protocol adopts the protocols.io Insulin Tolerance Test in Mouse v1. Mice are fasted for 4–6 hours, weighed, and given insulin intraperitoneally at 1–1.2 U/kg. Blood glucose is measured before and at 15, 30, 60, 90 and 120 minutes after injection. The measurement is a glucose assay (OBI:2100020); insulin administration, dose and time points belong to this concrete protocol and are not presented as an exact OBI insulin-tolerance-test term.

  • 小鼠口服葡萄糖耐量试验（OGTT）

    Version 2

    This protocol adopts the protocols.io Oral Glucose Tolerance Test in Mouse v1. Mice are fasted for 4–6 hours, weighed, and given oral glucose at 1 g/kg (0.5 g/kg after at least 4 weeks of high-fat diet). Blood glucose is measured before and at 15, 30, 60, 90 and 120 minutes after the load, with tail-vein plasma collected at selected time points for insulin measurement. The record describes the assay procedure, not executed disease-model results.

  • Hematoxylin and Eosin (H&E) staining for histopathology

    Version 5

    Hematoxylin and eosin (H&E) staining of fixed, paraffin-embedded tissue sections (4 μm). The workflow covers dewaxing, rehydration, hematoxylin nuclear staining, bluing, eosin counterstaining, dehydration and clearing, and coverslipping, yielding blue-purple nuclei and pink cytoplasm and connective tissue fibers for light-microscopic assessment of cellular and tissue morphology.

  • 主动脉 en face 斑块成像与面积定量

    Version 3

    Fixative-stored mouse aortas are cleaned, cut open, pinned and imaged with a mm ruler; regional intimal and lesion areas are traced and measured in Image Pro with blinded second-observer verification.

  • 小鼠腹腔注射葡萄糖耐量检测（IPGTT）

    Version 3

    Intraperitoneal glucose tolerance test (IPGTT) in mice: single-house for one week, weigh, fast for 6 hours, measure baseline glucose, inject 2 g/kg body weight of 20% D-glucose intraperitoneally, then collect blood at 15 to 120 minutes after injection. Used to assess glucose clearance in obesity and glucose-metabolism models.

  • FFPE 肾组织切片 PAS 染色

    Version 1

    FFPE kidney sections undergo xylene dewaxing, graded-alcohol hydration, 0.5% periodic-acid oxidation, Schiff development, Mayer hematoxylin counterstain, bluing, dehydration, clearing and Cytoseal mounting, then brightfield scanning at 20x.

  • 加速转棒行为测定

    Version 1

    An accelerating rotarod assay of motor coordination, balance, and motor learning in freely moving mice: ramp duration set to 00:05:00 with speed capped at 50 (instrument default 80) and a minimum of 5; each mouse runs 4 trials per day for 4 consecutive days, with up to 5 animals per group and up to 4 groups per run separated by roughly 15-20 minutes; a trial ends when the animal falls onto the paddle or completes two consecutive rotations, and the daily average time and average rotations across the 4 trials form the readout; the rod is cleaned with 70% ethanol between trials.

  • Hematoxylin and Eosin (H&E) staining for histopathology

    Version 1

    Hematoxylin and eosin (H&E) staining of fixed, paraffin-embedded tissue sections (4 μm). The workflow covers dewaxing, rehydration, hematoxylin nuclear staining, bluing, eosin counterstaining, dehydration and clearing, and coverslipping, yielding blue-purple nuclei and pink cytoplasm and connective tissue fibers for light-microscopic assessment of cellular and tissue morphology.