Histopathological section staining
Observe tissue morphology and lesions, including plaque section staining in atherosclerosis research.
Technical services
Histopathology
Examine tissue pathology with section staining and immunohistochemistry.
IHC
Immunohistochemistry to examine the distribution of target markers in tissue.
Molecular assays
Explore gene expression and protein measures with qPCR, ELISA and WB.
qPCR
Quantitative PCR analysis to measure target gene expression.
ELISA
Enzyme-linked immunosorbent analysis to measure target proteins in samples.
Western blot (WB)
Western blot analysis to examine target protein expression.
Multiomics
Explore molecular changes in tissues and cells through transcriptomic data in model research.
Single-cell transcriptomics
Analyze gene expression at single-cell resolution, including hippocampal tissue in Alzheimer’s disease model research.
Sample requirements, assay scope, deliverables and current availability are confirmed for each research project.
Experimental methods
Experimental protocols
Protocols apply a method to a defined measurement, with samples, procedures and quality controls. Available services are linked separately.
小鼠胰岛素耐量试验（ITT）
Version 4
This protocol adopts the protocols.io Insulin Tolerance Test in Mouse v1. Mice are fasted for 4–6 hours, weighed, and given insulin intraperitoneally at 1–1.2 U/kg. Blood glucose is measured before and at 15, 30, 60, 90 and 120 minutes after injection. The measurement is a glucose assay (OBI:2100020); insulin administration, dose and time points belong to this concrete protocol and are not presented as an exact OBI insulin-tolerance-test term.
小鼠腹腔注射葡萄糖耐量检测（IPGTT）
Version 5
Intraperitoneal glucose tolerance test (IPGTT) in mice: single-house for one week, weigh, fast for 6 hours, measure baseline glucose, inject 2 g/kg body weight of 20% D-glucose intraperitoneally, then collect blood at 15 to 120 minutes after injection. Used to assess glucose clearance in obesity and glucose-metabolism models.
小鼠口服葡萄糖耐量试验（OGTT）
Version 6
This protocol adopts the protocols.io Oral Glucose Tolerance Test in Mouse v1. Mice are fasted for 4–6 hours, weighed, and given oral glucose at 1 g/kg (0.5 g/kg after at least 4 weeks of high-fat diet). Blood glucose is measured before and at 15, 30, 60, 90 and 120 minutes after the load, with tail-vein plasma collected at selected time points for insulin measurement. The record describes the assay procedure, not executed disease-model results.
大鼠尾静脉血糖测定
Version 3
Measure rat tail-vein blood glucose using Roche Accutrend Plus test strips and meter.
天狼星红（Picrosirius red）胶原染色
Version 4
This protocol adopts the protocols.io Picrosirius red staining for histology v1. Formalin-fixed paraffin-embedded sections are dewaxed and rehydrated, optionally pre-treated with phosphotungstic acid and counterstained with Weigert haematoxylin, then stained with 0.1% Sirius Red F3BA in saturated picric acid, differentiated with acetic acid, dehydrated, cleared and mounted. Collagen appears red in bright field and birefringent under polarised light. The record describes staining and microscopic observation, not a complete fibrosis area or stage quantification.
Hematoxylin and Eosin (H&E) staining for histopathology
Version 6
Hematoxylin and eosin (H&E) staining of fixed, paraffin-embedded tissue sections (4 μm). The workflow covers dewaxing, rehydration, hematoxylin nuclear staining, bluing, eosin counterstaining, dehydration and clearing, and coverslipping, yielding blue-purple nuclei and pink cytoplasm and connective tissue fibers for light-microscopic assessment of cellular and tissue morphology.
小鼠腹腔注射葡萄糖耐量检测（IPGTT）
Version 4
Intraperitoneal glucose tolerance test (IPGTT) in mice: single-house for one week, weigh, fast for 6 hours, measure baseline glucose, inject 2 g/kg body weight of 20% D-glucose intraperitoneally, then collect blood at 15 to 120 minutes after injection. Used to assess glucose clearance in obesity and glucose-metabolism models.
小鼠胰岛素耐量试验（ITT）
Version 3
This protocol adopts the protocols.io Insulin Tolerance Test in Mouse v1. Mice are fasted for 4–6 hours, weighed, and given insulin intraperitoneally at 1–1.2 U/kg. Blood glucose is measured before and at 15, 30, 60, 90 and 120 minutes after injection. The measurement is a glucose assay (OBI:2100020); insulin administration, dose and time points belong to this concrete protocol and are not presented as an exact OBI insulin-tolerance-test term.
小鼠口服葡萄糖耐量试验（OGTT）
Version 5
This protocol adopts the protocols.io Oral Glucose Tolerance Test in Mouse v1. Mice are fasted for 4–6 hours, weighed, and given oral glucose at 1 g/kg (0.5 g/kg after at least 4 weeks of high-fat diet). Blood glucose is measured before and at 15, 30, 60, 90 and 120 minutes after the load, with tail-vein plasma collected at selected time points for insulin measurement. The record describes the assay procedure, not executed disease-model results.
动脉组织石蜡切片 H&E 染色
Version 2
Hematoxylin and eosin staining of paraffin arterial sections for histological morphology.